A. Western blot analysis of anti-IL1 beta RabMAb (Cat. #AJ1391a), dilution 1:20,000. (approximately 0.05ug/lane [0.5ug/200ul] of recombinant protein was loaded on the membrane)
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells
Tissue Specificity
A recombinant human IL-1 beta protein was used as an immunogen.
Cellular Localization
Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins
A. Western blot analysis of anti-IL1 beta RabMAb (Cat. #AJ1391a), dilution 1:20,000. (approximately 0.05ug/lane [0.5ug/200ul] of recombinant protein was loaded on the membrane)
Application Notes
WB:1:20000
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.