Application
Western blot analysis of IL1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IL1B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL1B; IL1F2; Interleukin-1 beta; Catabolin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL1F2 |
|---|---|
| Alternative Names | IL1B IL1F2 Interleukin-1 beta Catabolin |
| Molecular Weight(MW) | 30748 Da |
| Function | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells |
| Tissue Specificity | This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the Center region of human IL1B. |
| Cellular Localization | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins |
| Entrez Gene | 3553 |
|---|
Application
Western blot analysis of IL1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
