Application
Western blot analysis of IL27 (arrow) using rabbit polyclonal IL27 Antibody (C71) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.Application
IL-27 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL-27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
IL-27 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IL-27 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL17D; IL27; Interleukin-17D; Interleukin-27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL27 |
|---|---|
| Alternative Names | IL17D IL27 Interleukin-17D Interleukin-27 |
| Molecular Weight(MW) | 21893 Da |
| Function | Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells |
| Tissue Specificity | Expressed preferentially in adipose, skeletal muscle and CNS |
| Cellular Localization | Secreted. |
| Entrez Gene | 53342 |
|---|
Application
Western blot analysis of IL27 (arrow) using rabbit polyclonal IL27 Antibody (C71) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.
Application
IL-27 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL-27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
IL-27 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
