Application
Immunocytochemistry of IL-27 in EL4 with IL-27 antibody at 10 μg/mL.Application
Western blot analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | IL-27 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha |
| Product description | IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.1) Hunter CA. New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat. Rev. Immunol. 2005; 5:521-31. |
| Immunogen | IL-27 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-27. The immunogen is located within the first 50 amino acids of IL-27. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| IL-27 antibody can be used for the detection of IL-27 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL27 |
|---|---|
| Alternative Names | IL-27 Antibody: p28 IL30 IL-27 IL27A IL-27A IL27p28 Interleukin-27 subunit alpha p28 IL-27 subunit alpha |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 29 kDa |
| Sequence Similarities | 2) Cat. No. 17-207 - EL4 Cell Slide |
Application
Immunocytochemistry of IL-27 in EL4 with IL-27 antibody at 10 μg/mL.
Application
Western blot analysis of IL-27 in A-20 cell lysate with IL-27 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | IL-27 antibody can be used for the detection of IL-27 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-27 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-27 Antibody is supplied in PBS containing 0.02% sodium azide. |
