Application
Western blot analysis of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | IL-27 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha |
| Product description | IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.1) Pfanz S, Timans JC, Cheung J et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity 2002; 16:779-90. |
| Immunogen | IL-27 antibody was raised against a 14 amino acid synthetic peptide from near the center of human IL-27. The immunogen is located within amino acids 80 - 130 of IL-27. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| IL-27 antibody can be used for the detection of IL-27 by Western blot at 2 - 4 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL27 |
|---|---|
| Alternative Names | IL-27 Antibody: p28 IL30 IL-27 IL27A IL-27A IL27p28 Interleukin-27 subunit alpha p28 IL-27 subunit alpha |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (92%) |
Application
Western blot analysis of IL-27 in Daudi lysate with IL-27 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | IL-27 antibody can be used for the detection of IL-27 by Western blot at 2 - 4 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-27 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | IL-27 Antibody is supplied in PBS containing 0.02% sodium azide. |
