Application
Western blot analysis of IL1RN antibody (C-term) (Cat.# AP6503b) in HepG2 cell line lysates (35ug/lane). IL1RN (arrow) was detected using the purified Pab.Application
Western blot analysis of IL1RN (arrow) using rabbit polyclonal IL1RN Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2) (Origene Technologies).Application
IL1RN Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of IL1RN Antibody (C-term)(Cat#AP6503b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | IL1RN Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL1RN; IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; ICIL-1RA; IL1 inhibitor; INN=Anakinra; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL1F3 IL1RA |
|---|---|
| Alternative Names | IL1RN IL1F3 IL1RA Interleukin-1 receptor antagonist protein ICIL-1RA IL1 inhibitor INN=Anakinra Flags: Precursor |
| Molecular Weight(MW) | 20055 Da |
| Function | Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure |
| Tissue Specificity | The intracellular form of IL1RN is predominantly expressed in epithelial cells |
| Cellular Localization | Isoform 1: Secreted. Isoform 3: Cytoplasm. |
| Entrez Gene | 3557 |
|---|
Application
Western blot analysis of IL1RN antibody (C-term) (Cat.# AP6503b) in HepG2 cell line lysates (35ug/lane). IL1RN (arrow) was detected using the purified Pab.
Application
Western blot analysis of IL1RN (arrow) using rabbit polyclonal IL1RN Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2) (Origene Technologies).
Application
IL1RN Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of IL1RN Antibody (C-term)(Cat#AP6503b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
