Application
Western blot analysis of IL1RN (arrow) using rabbit polyclonal IL1RN Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2) (Origene Technologies).Application
IL1RN Antibody (Center) (RB18901) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL1RN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of IL1RN Antibody (Center)(Cat#AP6503a) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
IL1RN Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | IL1RN Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL1RN; IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; ICIL-1RA; IL1 inhibitor; INN=Anakinra; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | IL1F3 IL1RA |
|---|---|
| Alternative Names | IL1RN IL1F3 IL1RA Interleukin-1 receptor antagonist protein ICIL-1RA IL1 inhibitor INN=Anakinra Flags: Precursor |
| Molecular Weight(MW) | 20055 Da |
| Function | Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure |
| Tissue Specificity | The intracellular form of IL1RN is predominantly expressed in epithelial cells |
| Cellular Localization | Isoform 1: Secreted. Isoform 3: Cytoplasm. |
| Entrez Gene | 3557 |
|---|
Application
Western blot analysis of IL1RN (arrow) using rabbit polyclonal IL1RN Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2) (Origene Technologies).
Application
IL1RN Antibody (Center) (RB18901) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL1RN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of IL1RN Antibody (Center)(Cat#AP6503a) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
IL1RN Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
