Application
INA Antibody (Cat. #AM1916b) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).Application
Confocal immunofluorescent analysis of INA Antibody (Cat#AM1916b) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).Application
INA Antibody (Cat. #AM1916b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INA Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | INA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INA; NEF5; Alpha-internexin; 66 kDa neurofilament protein; Neurofilament 5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 257CT7.1.2 |
| Host Species | Mouse |
| Tested Applications | WBIFIHC |
| WB:1:100~250 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NEF5 |
|---|---|
| Alternative Names | INA NEF5 Alpha-internexin 66 kDa neurofilament protein Neurofilament 5 |
| Molecular Weight(MW) | 55391 Da |
| Function | Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges |
| Tissue Specificity | Found predominantly in adult CNS. |
| Entrez Gene | 9118 |
|---|---|
| Nucleotide Accession | NP_116116.1 |
Application
INA Antibody (Cat. #AM1916b) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the INA antibody detected the INA protein (arrow).
Application
Confocal immunofluorescent analysis of INA Antibody (Cat#AM1916b) with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
INA Antibody (Cat. #AM1916b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INA Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~250 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
