Application
Figure 1: Western blot analysis using anti-ISL1 monoclonal antibody against full-length ISL1(aa1-349)-hIgGFc transfected HEK293 cell lysate(1).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using anti-ISL1 antibody with DAB staining.Application
Figure 3: Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using anti-ISL1 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.| Product Name | ISL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Isl-1; ISLET1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H9 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Isl-1 ISLET1 |
|---|---|
| Molecular Weight(MW) | 39036 Da |
| Function | Binds to one of the cis-acting domain of the insulin gene enhancer |
| Tissue Specificity | Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain |
| Cellular Localization | Nucleus. |
| Entrez Gene | 3670 |
|---|---|
| Nucleotide Accession | NP_002193.2 |
Application
Figure 1: Western blot analysis using anti-ISL1 monoclonal antibody against full-length ISL1(aa1-349)-hIgGFc transfected HEK293 cell lysate(1).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using anti-ISL1 antibody with DAB staining.
Application
Figure 3: Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using anti-ISL1 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human ISL1 expressed in E. Coli. |
