Application
Western blot analysis of ISG15(arrow) using rabbit polyclonal ISG15 Antibody (Center R87) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with ISG15 gene (Lane 2) (Origene Technologies).| Product Name | ISG15 Antibody (Center R87) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ISG15; G1P2; UCRP; Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; Ubiquitin cross-reactive protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G1P2 UCRP |
|---|---|
| Alternative Names | ISG15 G1P2 UCRP Ubiquitin-like protein ISG15 Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Ubiquitin cross-reactive protein |
| Molecular Weight(MW) | 17888 Da |
| Function | Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections |
| Tissue Specificity | Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons |
| Cellular Localization | Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted |
| Entrez Gene | 9636 |
|---|
Application
Western blot analysis of ISG15(arrow) using rabbit polyclonal ISG15 Antibody (Center R87) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with ISG15 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
