Application
Western blot analysis of anti-ITA6 (Cat. #AM1828a) in HepG2 cell line lysates (35ug/lane). ITA6 (arrow) was detected using the purified Mab.(8ug/ml)Application
ITA6 Monoclonal Antibody (Cat. #AM1828a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ITA6 Antibody (Cat#AM1828a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).Application
ITA6 Monoclonal Antibody (Cat. #AM1828a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Product Name | ITA6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ITGA6; Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD_antigen=CD49f; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 129CT49.6.5 |
| Host Species | Mouse |
| Tested Applications | WBIHCIFFC |
| WB:1:100-16000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ITGA6 Integrin alpha-6 CD49 antigen-like family member F VLA-6 CD_antigen=CD49f Integrin alpha-6 heavy chain Integrin alpha-6 light chain |
|---|---|
| Molecular Weight(MW) | 126633 Da |
| Function | Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome |
| Tissue Specificity | Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 3655 |
|---|
Application
Western blot analysis of anti-ITA6 (Cat. #AM1828a) in HepG2 cell line lysates (35ug/lane). ITA6 (arrow) was detected using the purified Mab.(8ug/ml)
Application
ITA6 Monoclonal Antibody (Cat. #AM1828a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ITA6 Antibody (Cat#AM1828a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
ITA6 Monoclonal Antibody (Cat. #AM1828a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.| Application Notes | WB:1:100-16000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
