Application
Figure 1: Western blot analysis using anti-JAK3 mAb against Jurkat cell lysate (1).Application
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Product Name | JAK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JAKL; LJAK |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5H2 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | JAKL LJAK |
|---|---|
| Molecular Weight(MW) | 125099 Da |
| Function | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion |
| Tissue Specificity | In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins |
| Cellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). Cytoplasm (By similarity) |
| Entrez Gene | 3718 |
|---|---|
| Nucleotide Accession | NP_000206.2 |
Application
Figure 1: Western blot analysis using anti-JAK3 mAb against Jurkat cell lysate (1).
Application
Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human JAK3 expressed in E. Coli. |
