Application
Figure 1: Western blot analysis using anti-KARS monoclonal antibody against truncated Trx-KARS recombinant protein(1), truncated MBP-KARS(aa90-174) and full length KARS(aa1-188) transfected CHO-K1 cell lysate(3).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using anti-KARS antibody.| Product Name | KARS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KRS; KARS2; KIAA0070 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8G12C1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0070 |
|---|---|
| Alternative Names | KRS KARS2 KIAA0070 |
| Molecular Weight(MW) | 68048 Da |
| Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation |
| Tissue Specificity | Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli. |
| Cellular Localization | Isoform Cytoplasmic: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted Note=Secretion is induced by TNF-alpha |
| Entrez Gene | 3735 |
|---|---|
| Nucleotide Accession | NP_005539.1 |
Application
Figure 1: Western blot analysis using anti-KARS monoclonal antibody against truncated Trx-KARS recombinant protein(1), truncated MBP-KARS(aa90-174) and full length KARS(aa1-188) transfected CHO-K1 cell lysate(3).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using anti-KARS antibody.| Application Notes | WB:1:500 - 2000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli. |
