Application
Western blot analysis of KCNIP3 (arrow) using rabbit polyclonal KCNIP3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KCNIP3 gene.Application
KCNIP3 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KCNIP3 antibody detected the KCNIP3 protein (arrow).Application
Confocal immunofluorescent analysis of KCNIP3 Antibody (C-term)(Cat#AP13839b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
KCNIP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCNIP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | KCNIP3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KCNIP3; CSEN; DREAM; KCHIP3; Calsenilin; A-type potassium channel modulatory protein 3; DRE-antagonist modulator; Kv channel-interacting protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CSEN DREAM KCHIP3 |
|---|---|
| Alternative Names | KCNIP3 CSEN DREAM KCHIP3 Calsenilin A-type potassium channel modulatory protein 3 DRE-antagonist modulator Kv channel-interacting protein 3 |
| Molecular Weight(MW) | 29231 Da |
| Function | Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity) May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation |
| Tissue Specificity | Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease |
| Cellular Localization | Cytoplasm. Cell membrane; Lipid-anchor (By similarity). Endoplasmic reticulum. Golgi apparatus. Nucleus Note=Also membrane-bound, associated with the plasma membrane (By similarity). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus |
| Entrez Gene | 30818 |
|---|---|
| Nucleotide Accession | NP_001030086.1 |
Application
Western blot analysis of KCNIP3 (arrow) using rabbit polyclonal KCNIP3 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KCNIP3 gene.
Application
KCNIP3 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KCNIP3 antibody detected the KCNIP3 protein (arrow).
Application
Confocal immunofluorescent analysis of KCNIP3 Antibody (C-term)(Cat#AP13839b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
KCNIP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCNIP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
