Application
KEAP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the KEAP1 antibody detected the KEAP1 protein (arrow).Application
KEAP1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KEAP1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | KEAP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; Kelch-like protein 19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | INRF2 KIAA0132 KLHL19 |
|---|---|
| Alternative Names | KEAP1 INRF2 KIAA0132 KLHL19 Kelch-like ECH-associated protein 1 Cytosolic inhibitor of Nrf2 Kelch-like protein 19 |
| Molecular Weight(MW) | 69666 Da |
| Function | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome |
| Tissue Specificity | Broadly expressed, with highest levels in skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus |
| Entrez Gene | 9817 |
|---|---|
| Nucleotide Accession | NP_036421.2 NP_987096.1 |
Application
KEAP1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the KEAP1 antibody detected the KEAP1 protein (arrow).
Application
KEAP1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KEAP1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
