Application
KEAP1 Antibody (Cat. #AM1968b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the KEAP1 antibody detected the KEAP1 protein (arrow).| Product Name | KEAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; Kelch-like protein 19 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 297CT6.1.6 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | INRF2 KIAA0132 KLHL19 |
|---|---|
| Alternative Names | KEAP1 INRF2 KIAA0132 KLHL19 Kelch-like ECH-associated protein 1 Cytosolic inhibitor of Nrf2 Kelch-like protein 19 |
| Molecular Weight(MW) | 69666 Da |
| Function | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome |
| Tissue Specificity | Broadly expressed, with highest levels in skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus |
| Entrez Gene | 9817 |
|---|---|
| Nucleotide Accession | NP_036421.2 NP_987096.1 |
Application
KEAP1 Antibody (Cat. #AM1968b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the KEAP1 antibody detected the KEAP1 protein (arrow).| Application Notes | WB:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS. |
