Application
KIRREL Antibody (Center) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the KIRREL antibody detected the KIRREL protein (arrow).Application
KIRREL antibody(Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIRREL antibody(Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KIRREL Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KIRREL Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIRREL; KIRREL1; NEPH1; Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Nephrin-like protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIRREL1 NEPH1 |
|---|---|
| Alternative Names | KIRREL KIRREL1 NEPH1 Kin of IRRE-like protein 1 Kin of irregular chiasm-like protein 1 Nephrin-like protein 1 |
| Molecular Weight(MW) | 83536 Da |
| Function | Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1 (By similarity) |
| Tissue Specificity | Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Note=Predominantly located at podocyte slit diaphragm |
| Entrez Gene | 55243 |
|---|---|
| Nucleotide Accession | NP_060710.3 |
Application
KIRREL Antibody (Center) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the KIRREL antibody detected the KIRREL protein (arrow).
Application
KIRREL antibody(Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIRREL antibody(Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KIRREL Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
