Application
Western blot analysis of KNG1 (arrow) using rabbit polyclonal KNG1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KNG1 gene.Application
KNG1 Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the KNG1 antibody detected the KNG1 protein (arrow).Application
KNG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KNG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
KNG1 Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KNG1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KNG1; BDK; KNG; Kininogen-1; Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; Williams-Fitzgerald-Flaujeac factor; Kininogen-1 heavy chain; T-kinin; Ile-Ser-Bradykinin; Bradykinin; Kallidin I; Lysyl-bradykinin; Kallid |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BDK KNG |
|---|---|
| Alternative Names | KNG1 BDK KNG Kininogen-1 Alpha-2-thiol proteinase inhibitor Fitzgerald factor High molecular weight kininogen Williams-Fitzgerald-Flaujeac factor Kininogen-1 heavy chain T-kinin Ile-Ser-Bradykinin Bradykinin Kallidin I Lysyl-bradykinin Kallid |
| Molecular Weight(MW) | 71957 Da |
| Function | (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting |
| Tissue Specificity | Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors |
| Cellular Localization | Secreted, extracellular space. |
| Entrez Gene | 3827 |
|---|---|
| Nucleotide Accession | NP_001095886.1 NP_000884.1 |
Application
Western blot analysis of KNG1 (arrow) using rabbit polyclonal KNG1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KNG1 gene.
Application
KNG1 Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the KNG1 antibody detected the KNG1 protein (arrow).
Application
KNG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KNG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
KNG1 Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM236768