Application
Western blot analysis of KPNA3 Antibody (N-term) in CEM cell line lysates (35ug/lane).KPNA3 (arrow) was detected using the purified Pab.Application
Western blot analysis of KPNA3 (arrow) using rabbit polyclonal KPNA3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KPNA3 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with GNAL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
KPNA3 Antibody (N-term) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | KPNA3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPNA3; QIP2; Importin subunit alpha-3; Importin alpha Q2; Karyopherin subunit alpha-3; SRP1-gamma |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | QIP2 |
|---|---|
| Alternative Names | KPNA3 QIP2 Importin subunit alpha-3 Importin alpha Q2 Karyopherin subunit alpha-3 SRP1-gamma |
| Molecular Weight(MW) | 57811 Da |
| Function | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9 |
| Tissue Specificity | Ubiquitous. Highest levels in heart and skeletal muscle |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 3839 |
|---|
Application
Western blot analysis of KPNA3 Antibody (N-term) in CEM cell line lysates (35ug/lane).KPNA3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of KPNA3 (arrow) using rabbit polyclonal KPNA3 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KPNA3 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with GNAL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
KPNA3 Antibody (N-term) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
