Application
LAMP2 Antibody (Cat. #AM1851b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the LAMP2 antibody detected the LAMP2 protein (arrow).Application
Confocal immunofluorescent analysis of LAMP2 Antibody (Cat#AM1851b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | LAMP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LAMP2; Lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B; CD_antigen=CD107b; Flags: Precursor |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 157CT16.6.6 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:100~1000 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | LAMP2 Lysosome-associated membrane glycoprotein 2 CD107 antigen-like family member B CD_antigen=CD107b Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 44961 Da |
| Function | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens |
| Tissue Specificity | Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane |
| Entrez Gene | 3920 |
|---|---|
| Nucleotide Accession | NP_054701.1 |
Application
LAMP2 Antibody (Cat. #AM1851b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the LAMP2 antibody detected the LAMP2 protein (arrow).
Application
Confocal immunofluorescent analysis of LAMP2 Antibody (Cat#AM1851b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~1000 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
