Application
Western blot analysis of APG8b (MAP1LC3B) Antibody (T6) (Cat.# AP1802d) in Y79 cell line lysates and mouse brain tissue lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.Application
Western blot analysis of MAP1LC3B (arrow) using rabbit polyclonal APG8b (MAP1LC3B) Antibody (T6) (Cat.# AP1802d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP1LC3B gene (Lane 2) (Origene Technologies).| Product Name | LC3 Antibody (APG8B) (T6) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 lig |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAP1ALC3 |
|---|---|
| Alternative Names | MAP1LC3B MAP1ALC3 Microtubule-associated proteins 1A/1B light chain 3B Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Microtubule-associated protein 1 lig |
| Molecular Weight(MW) | 14688 Da |
| Function | Involved in formation of autophagosomal vacuoles (autophagosomes) |
| Tissue Specificity | Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
| Entrez Gene | 81631 |
|---|
Application
Western blot analysis of APG8b (MAP1LC3B) Antibody (T6) (Cat.# AP1802d) in Y79 cell line lysates and mouse brain tissue lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.
Application
Western blot analysis of MAP1LC3B (arrow) using rabbit polyclonal APG8b (MAP1LC3B) Antibody (T6) (Cat.# AP1802d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP1LC3B gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
