Application
Western blot analysis of LPIN1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).LPIN1 (arrow) was detected using the purified Pab.Application
LPIN1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LPIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
LPIN1 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LPIN1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0188 |
|---|---|
| Alternative Names | LPIN1 KIAA0188 Phosphatidate phosphatase LPIN1 Lipin-1 |
| Molecular Weight(MW) | 98664 Da |
| Function | Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity) |
| Tissue Specificity | Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract |
| Cellular Localization | Nucleus membrane (By similarity). Cytoplasm, cytosol (By similarity). Endoplasmic reticulum membrane (By similarity) |
| Entrez Gene | 23175 |
|---|
Application
Western blot analysis of LPIN1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane).LPIN1 (arrow) was detected using the purified Pab.
Application
LPIN1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LPIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
LPIN1 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
