| Product Name | 14-3-3 (phospho Ser) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3i2] to 14 - 3 - 3 Binding Motif ( phospho S )This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery. |
| Immunogen | Synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled). |
| Modification | p-Ser |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3i2 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| ELISA: Use at a dilution of 1/1000. IP: Use at a dilution of 1/20 on denatured proteins. WB: Use at a dilution of 1/4000. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 14-3-3 (phospho S) 14-3-3 (phospho Ser) 14-3-3 (pS) 14-3-3 (pSer) 14-3-3 phospho S 14-3-3 phospho Ser Phospho 14-3-3 (S) Phospho 14-3-3 (Ser) Phospho 14-3-3 (pS) Phospho 14-3-3 (pSer) |
|---|---|
| Target | 14-3-3 binding motif |
| Tissue Specificity | binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA the antibody recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences. |
| Cellular Localization | Cytoplasmic |
| Application Notes | ELISA: Use at a dilution of 1/1000. IP: Use at a dilution of 1/20 on denatured proteins. WB: Use at a dilution of 1/4000. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap |
| Storage Buffer | Preservative: 0.02% sodium azide; Constituents: 50% Glycerol, BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5 |
