Application
Western blot analysis probed with Lane 1: Human HeLa whole extract Lane 2: Mouse brain extract Lane 3: Rat brain extract| Product Name | 14-3-3 beta + epsilon + zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3C8] to 14 - 3 - 3 beta + epsilon + zeta14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors. |
| Immunogen | Human 14-3-3 beta fusion protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3C8 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 1 - 10 μg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 1433 beta + epsilon + zeta 1433 GW128 14-3-3E 14-3-3-zeta HS1 FLJ45465 KCIP-1 FLJ53559 MGC111427 YWHAA MGC126532 MDCR MGC138156 MDS YWHAD KCIP 1 14 3 3 zeta KCIP1 1433zeta |
|---|---|
| Target | 14-3-3 beta + epsilon + zeta |
| Tissue Specificity | This antibody detects an ~30 kDa protein, corresponding to the apparent molecular mass of 14-3-3 beta, 14-3-3 epsilon or 14-3-3 zeta on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. |
| Cellular Localization | Cytoplasmic |
Application
Western blot analysis probed with Lane 1: Human HeLa whole extract Lane 2: Mouse brain extract Lane 3: Rat brain extract| Positive Control | HeLa Cell Lysate, Mouse Brain Tissue Extract, Rat Brain Tissue Extract |
|---|---|
| Application Notes | WB: Use at a concentration of 1 - 10 μg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: 50% Glycerol, PBS. pH 7.2 |
