Application
Western blot analysis of mouse brain tissue extract, probed with Lane 1: Mouse IgG Lane 2: Anti-14-3-3β| Product Name | 14-3-3 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4E1] to 14 - 3 - 3 beta14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasma of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors. |
| Immunogen | Recombinant full length protein (Rat). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4E1 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 1 - 5 μg/ml. Detects a band of approximately 29 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | KCIP-1 KCIP1 1433 YWHAB HS1 P31946 GW128 1433 beta 601289 7529 KCIP 1 |
|---|---|
| Target | 14-3-3 beta |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Western blot analysis of mouse brain tissue extract, probed with Lane 1: Mouse IgG Lane 2: Anti-14-3-3β| Positive Control | Mouse brain tissue extract. |
|---|---|
| Application Notes | IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 1 - 5 μg/ml. Detects a band of approximately 29 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 1mg/ml Sodium Azide; Constituents: PBS, 10mg/ml Sucrose. pH 7.4 |
