| Product Name | 14-3-3 sigma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1.N.6] to 14 - 3 - 3 sigma - BSA and Azide freeMembers of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 1.N.6 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a concentration of 2 - 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 28 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | P31947 601290 1433 1433 sigma SFN 2810 |
|---|---|
| Target | 14-3-3 sigma |
| Tissue Specificity | Does not cross-react with other 14-3-3 isoforms. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | HT29 cell lysate and skin tissue slides |
|---|---|
| Application Notes | IHC-P: Use at a concentration of 2 - 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 28 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | May be stored at 4ºC for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap |
| Storage Buffer | Preservative: None; Constituents: PBS. pH 7.4 |
