| Product Name | 14-3-3 sigma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1.T.28] to 14 - 3 - 3 sigmaMembers of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions. |
| Immunogen | Fusion protein corresponding to full-length human 14-3-3 sigma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1.T.28 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 0.5 µg/ml. GTX14122 detects 20µg of reduced EGF-stimulated A431 cell lysate, visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | P31947 601290 1433 1433 sigma SFN 2810 |
|---|---|
| Target | 14-3-3 sigma |
| Tissue Specificity | recognizes 14-3-3 sigma and does not crossreact with other 14-3-3 isoforms. |
| Cellular Localization | Cytoplasmic |
| Positive Control | EGF-stimulated A431 cell lysate |
|---|---|
| Application Notes | WB: Use at a concentration of 0.5 µg/ml. GTX14122 detects 20µg of reduced EGF-stimulated A431 cell lysate, visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 30 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4 |
