Application
MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled with Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD), clone 58K-9 (Cat. No. ) at a 1:100 dilution.Application
HeLa cells fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS. Stained with anti-58K Golgi protein monoclonal antibody (1:50) followed by anti-Mouse IgG conjugated to Cy3 (1:100).Application
IF staining was performed on COS7 cells using a 1:100 dilution of the primary antibody followed by hybridisation with a 1:100 dilution of the secondary anti-mouse antibody R270 with red fluorescence. Picture from review by Karin Birkenkamp-Demtroeder submitted 20 July 2004.Application
Formalin-fixed parafin embedded colon was incubated with at range of dilutions (1/100 - 1/3000). Excellent staining of the colonic epithelium was seen using 1/1000 dilution. Heat-induced epitope retrieval was used.Application
Cells were grown on coverslips, fixed with 70% methanol, and permeabilized with 0.5% Triton X-100 then double labeled by incubation with a rabbit polyclonal antibody against HE4 (a secreted ovarian cancer biomarker) and monoclonal antibodies against the 5Application
Formalin-fixed parafin embedded ovary was incubated with at range of dilutions (1/100 - 1/3000). Excellent staining of the Golgi in Ovarian inclusion cysts was seen using 1/1000 dilution. Heat-induced epitope retrieval was used.| Product Name | 58K Golgi protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [58K - 9] to 58K Golgi protein - Golgi Marker58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. |
| Immunogen | Full length native protein (purified) (Rat liver). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 58K-9 |
| Host Species | Mouse |
| Tested Applications | EMICC/IFIHCIHC-PWB |
| FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available.IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanDogPigRatHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 89833 58K Golgi protein Ftcd |
|---|---|
| Target | 58K Golgi protein |
| Tissue Specificity | The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. |
| Cellular Localization | Cytoplasmic and Golgi Apparatu |
Application
MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled with Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD), clone 58K-9 (Cat. No. ) at a 1:100 dilution.
Application
HeLa cells fixed and permeabilized with 3% PFA plus 0.05% Triton X-100 in PBS. Stained with anti-58K Golgi protein monoclonal antibody (1:50) followed by anti-Mouse IgG conjugated to Cy3 (1:100).
Application
IF staining was performed on COS7 cells using a 1:100 dilution of the primary antibody followed by hybridisation with a 1:100 dilution of the secondary anti-mouse antibody R270 with red fluorescence. Picture from review by Karin Birkenkamp-Demtroeder submitted 20 July 2004.
Application
Formalin-fixed parafin embedded colon was incubated with at range of dilutions (1/100 - 1/3000). Excellent staining of the colonic epithelium was seen using 1/1000 dilution. Heat-induced epitope retrieval was used.
Application
Cells were grown on coverslips, fixed with 70% methanol, and permeabilized with 0.5% Triton X-100 then double labeled by incubation with a rabbit polyclonal antibody against HE4 (a secreted ovarian cancer biomarker) and monoclonal antibodies against the 5
Application
Formalin-fixed parafin embedded ovary was incubated with at range of dilutions (1/100 - 1/3000). Excellent staining of the Golgi in Ovarian inclusion cysts was seen using 1/1000 dilution. Heat-induced epitope retrieval was used.| Positive Control | For indirect immunofluorescence: cultured Chinese hamster ovary (CHO) cells For immunoblotting (colorimetric): whole rat liver extractAntigen MW: 58 kDa |
|---|---|
| Application Notes | FuncS: Use at an assay dependent dilution. ICC: 1/50. Recommended protocol available.IF: 1/250. IM: Use at an assay dependent dilution. WB: 1/5000. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid with 15mM sodium azide |
