Application
6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody . Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG with HRP substrates.Application
Sample A: 10 µg of non-transformed E. coli lysate B: 10 µg of non-transformed E. coli lysate, induced C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced 12% SDS PAGE diluated at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody . The precipitated His-tagged protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent.Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | 6X His tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to 6X His tag |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-WIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:2000 IP:1:100~1:500 |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 6X His tag antibody His antibody 6x His antibody 6x his tag antibody His Tag antibody 6xHis Tag antibody 6 x His antibody |
|---|
Application
6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody . Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG with HRP substrates.
Application
Sample A: 10 µg of non-transformed E. coli lysate B: 10 µg of non-transformed E. coli lysate, induced C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced 12% SDS PAGE diluated at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody . The precipitated His-tagged protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent.
Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | His-tagged recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:2000 IP:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
