Application
6X His tag antibody [GT359] detects His-recombinant protein by immunofluorescent analysis. Sample: His-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: His-recombinant protein stained by 6X His tag antibody [GT359] diluted at 1:2000. Blue: Hoechst 33342 staining.Application
6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.Application
6X His tag antibody [GT359] detects 6xHis Tag protein by western blot analysis. A. 2 µg GST-recombinant protein B. 2 µg His-recombinant protein C. 1 µg His-recombinant protein D. 0.5 µg His-recombinant protein E. 0.1 µg His-recombinant protein F. 0.05 µg His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:3000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
6X His tag antibody [GT359] detects 6x His protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
6X His tag antibody [GT359] detects 6x His protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments. IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] diluted at 1:1000.| Product Name | 6X His tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse Monoclonal antibody [GT359] to 6X His tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | GT359 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 6X His tag antibody His antibody 6x His antibody 6x his tag antibody His Tag antibody 6xHis Tag antibody 6 x His antibody |
|---|
Application
6X His tag antibody [GT359] detects His-recombinant protein by immunofluorescent analysis. Sample: His-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: His-recombinant protein stained by 6X His tag antibody [GT359] diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] . Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG with HRP substrates.
Application
6X His tag antibody [GT359] detects 6xHis Tag protein by western blot analysis. A. 2 µg GST-recombinant protein B. 2 µg His-recombinant protein C. 1 µg His-recombinant protein D. 0.5 µg His-recombinant protein E. 0.1 µg His-recombinant protein F. 0.05 µg His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:3000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
6X His tag antibody [GT359] detects 6x His protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
6X His tag antibody [GT359] detects 6x His protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT359] dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments. IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] diluted at 1:1000.| Positive Control | His-recombinant protein , IPTG-induced cell lysate from E. coli expressing His-tagged proteins |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
