Application
His-ubiquitin protein probed with| Product Name | 6X His tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal [3H2201] to 6X His tagGTX15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Immunogen | PAX6 transcription factor linked to 6-histidine tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H2201 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| For ELISA: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:250-1:500. Optimal dilutions/concentrations should be determined by the end user: |
|
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Target | 6X His tag |
|---|---|
| Tissue Specificity | Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. In Western Blot of bacterial extracts, the antibody does not crossreact with any endogenous products. |
Application
His-ubiquitin protein probed with| Application Notes | For ELISA: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:250-1:500. Optimal dilutions/concentrations should be determined by the end user: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH7.2 containing 0.1% sodium azide and 40% glycerol |
