Application
Western blot analysis of AAK1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.| Product Name | AAK1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AAK1 (N-term)Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 194~223 amino acids from the n-terminal region of human AAK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Adaptorassociated kinase1 Adaptorassociated kinase 1 KIAA1048 AAK1 Adaptor-associated kinase 1 AP2associated protein kinase 1 AP2-associated protein kinase 1 AP2associated protein kinase1 Adaptor associated kinase 1 |
|---|---|
| Target | AAK1 |
| Molecular Weight(MW) | 93.578 kDa (note) |
| Cellular Localization | Active when found in clathrin-coated pits at the plasma membrane. |
Application
Western blot analysis of AAK1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). AAK1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
