Application
Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01). Lane B: Immunostaining with anti-EGFP (polyclonal). Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)| Product Name | ABRA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [ABRA1-01] to ABRA1ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.Entrez Gene: 4q21.23 |
| Immunogen | Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | ABRA1-01 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| FACS: Use at 1-2 µg/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLJ11520 FLJ12642 |
|---|---|
| Target | ABRA1 |
| Tissue Specificity | The mouse monoclonal antibody ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions. |
| Cellular Localization | Localizes at sites of DNA damage at double-strand breaks. |
Application
Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01). Lane B: Immunostaining with anti-EGFP (polyclonal). Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)| Application Notes | FACS: Use at 1-2 µg/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
