Application
Sample (30 ug of whole cell lysate) A: A549 B: Hela 10% SDS PAGE diluted at 1:1000Application
ACAA1 antibody detects ACAA1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACAA1 protein stained by ACAA1 antibody diluted at 1:250. Blue: Hoechst 33342 staining.Application
Sample (50 ug of whole cell lysate) A: mouse Liver 10% SDS PAGE diluted at 1:1000Application
ACAA1 antibody detects ACAA1 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ACAA1 antibody dilution: 1:500.| Product Name | ACAA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACAA1 (acetyl-CoA acyltransferase 1)This gene encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ACAA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAA antibody PTHIO antibody THIO antibody ACAA1 antibody acetyl-Coenzyme A acyltransferase 1 antibody peroxisomal 3-oxoacyl-CoA thiolase antibody beta-ketothiolase antibody 3-ketoacyl-CoA thiolase peroxisomal antibody peroxisomal 3-oxoacyl-Coenzy |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (2) |
| Cellular Localization | Peroxisome |
Application
Sample (30 ug of whole cell lysate) A: A549 B: Hela 10% SDS PAGE diluted at 1:1000
Application
ACAA1 antibody detects ACAA1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACAA1 protein stained by ACAA1 antibody diluted at 1:250. Blue: Hoechst 33342 staining.
Application
Sample (50 ug of whole cell lysate) A: mouse Liver 10% SDS PAGE diluted at 1:1000
Application
ACAA1 antibody detects ACAA1 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ACAA1 antibody dilution: 1:500.| Positive Control | A549 , HeLa , mouse Liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
