Application
ACADM antibody detects ACADM protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ACADM antibody dilution: 1:1000Application
ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADM protein stained by ACADM antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
ACADM antibody detects ACADM protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE ACADM antibody dilution: 1:1000Application
Sample(30 μg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:2000Application
ACADM antibody detects ACADM protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ACADM antibody dilution: 1:250.| Product Name | ACADM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ACADM (acyl-CoA dehydrogenase, C-4 to C-12 straight chain)This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ACADM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chimpanzee, BovineZebrafish (87%), Xenopus laevis (87%), Pig (91%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACAD1 antibody FLJ18227 antibody FLJ93013 antibody FLJ99884 antibody MCAD antibody MCADH antibody ACADM antibody acyl-Coenzyme A dehydrogenase C-4 to C-12 straight chain antibody medium-chain specific acyl-CoA dehydrogenase mitochondrial antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (4) |
| Cellular Localization | Mitochondrion matrix |
Application
ACADM antibody detects ACADM protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ACADM antibody dilution: 1:1000
Application
ACADM antibody detects ACADM protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACADM protein stained by ACADM antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
ACADM antibody detects ACADM protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE ACADM antibody dilution: 1:1000
Application
Sample(30 μg of whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:2000
Application
ACADM antibody detects ACADM protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ACADM antibody dilution: 1:250.| Positive Control | Molt-4 , Neuro2A , GL261 , C8D30 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
