Application
WB analysis of indicated lysates (40ug per lane) using ACAT1 antibody at a dilution of 1:1,000.| Product Name | ACAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT15E5] to ACAT1This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009] |
| Immunogen | Recombinant human ACAT1 (34-427aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT15E5 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | acetyl-CoA acetyltransferase 1 THIL ACAT MAT T2 |
|---|---|
| Target | ACAT1 |
| Light Chain Type | kappa |
Application
WB analysis of indicated lysates (40ug per lane) using ACAT1 antibody at a dilution of 1:1,000.| Application Notes | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.01% Sodium Azide. |
