Application
Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ACAT2Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.Application
HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.Application
Flow cytometric Analysis of Jurkat cells, using anti-ACAT2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.Application
Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody .| Product Name | ACAT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C5] to ACAT2The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1C5 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cytosolic acetoacetyl-CoA thiolase acetylCoenzyme A acetyltransferase2 acetoacetyl Coenzyme A thiolase OTTHUMP00000017527 acetylCoenzyme A acetyltransferase 2 acetyl-Coenzyme A acetyltransferase 2 ACAT2 cytosolic acetoacetylCoA thiolase |
|---|---|
| Target | ACAT2 |
| Tissue Specificity | is specific for human ACAT2 (NP_0058826). |
| Cellular Localization | Cytoplasm |
Application
Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ACAT2
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
Application
HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.
Application
Flow cytometric Analysis of Jurkat cells, using anti-ACAT2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
Application
Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Immunofluorescent staining of HeLa cells using anti-ACAT2 mouse monoclonal antibody .| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
