Application
Western blot analysis of ACCN1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ACCN1 (arrow) was detected using the purified Pab.Application
ACCN1 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.| Product Name | ACCN1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ACCN1 (Internal)This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and ACCN3 (variant 1) has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 127~156 amino acids from the Center region of human ACCN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Brain sodium channel 1 BNaC1 Amiloridesensitive cation channel 1 neuronal Acidsens Amiloridesensitive cation channel1 neuronal Acid-sensing ion channel 2 ASIC2 MDEG BNAC1 ACCN ACCN1 ACCN1 Amiloride-sensitive cation channel 1 neuronal Amilor |
|---|---|
| Target | ACCN1 |
| Molecular Weight(MW) | 57.709 kDa (note) |
| Cellular Localization | Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes. |
Application
Western blot analysis of ACCN1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ACCN1 (arrow) was detected using the purified Pab.
Application
ACCN1 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.| Application Notes | ELISA: Use at 1:1,000: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
