Application
Western blot analysis of ACCN2 Antibody (C-term) in CEM cell line lysates (35ug/lane). ACCN2 (arrow) was detected using the purified Pab.Application
ACCN2 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.| Product Name | ACCN2 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ACCN2 (C-term)This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 499~528 amino acids from the C-terminal region of human ACCN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Brain sodium channel2 BNaC2 Acidsensing ion channel 1 ASIC1 Amiloridesensitive cation channel2 neuronal BNAC2 ASIC1 ACCN2 Amiloride-sensitive cation channel 2 neuronal Acid-sensing ion channel 1 ASIC1 Acidsensing ion channel1 ASIC1 Brain sod |
|---|---|
| Target | ACCN2 |
| Molecular Weight(MW) | 59.967 kDa (note) |
| Cellular Localization | Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4. |
Application
Western blot analysis of ACCN2 Antibody (C-term) in CEM cell line lysates (35ug/lane). ACCN2 (arrow) was detected using the purified Pab.
Application
ACCN2 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
