| Product Name | Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2Q1055] to ActinThe two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Immunogen | Chicken gizzard actin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2Q1055 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-P |
| ELISA: Use at an assay dependent dilution. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. ICC: Use at a concentration of 10 μg/ml on methanol fixed mouse 3T3 cells. IHC-P: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 1 - 20 μg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanChicken |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Target | Actin |
|---|---|
| Tissue Specificity | recongizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms, it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. labels myotubes and stains myoblasts and fibroblasts, it does not interfer with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins, the antibody also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Application Notes | ELISA: Use at an assay dependent dilution. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. ICC: Use at a concentration of 10 μg/ml on methanol fixed mouse 3T3 cells. IHC-P: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 1 - 20 μg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 40% Glycerol, 10mM PBS. pH 7.6 |
