Application
Tissue stained with conserved C-terminus specific Monoclonal Anti-Actin . Indirect immunofluorescence staining of human tongue frozen section with Monoclonal Anti-Actin, Clone No. AC-40 .Application
ICC/IF analysis of Rat2 cells using Actin antibody [AC-40] at a dilution of 1:250 (red) and DAPI (blue).Application
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at a dilution of 1:500.Application
Immunohistochemical analysis of Agarose-embedded zebrafish embryo tail section , using Actin antibody at 1:200 dilution.| Product Name | Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [AC - 40] to ActinThe two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Immunogen | Synthetic peptide: SGPSIVHKCF (human) attached to Multiple Antigen Peptide (MAP) backbone. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AC-40 |
| Host Species | Mouse |
| Tested Applications | DotELISAICC/IFIHC-FIHC-PWBIB |
| Dot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IHC-F: Use at an assay dependent dilution. IF: Use at a dilution of 1/2000 determined by indirect immunofluorescent staining of cultured human or chicken fibroblasts. IHC-P: Use at an assay dependent dilution. Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel?s or Bouin?s solution may also be used as fixatives. IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/500 using cultured human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogPigRatZebra FishCarpGoatGuinea PigHamsterRabbitSheepBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 102610 ASMA NEM1 ACTA Actin NEM2 P68133 58 ACTA1 NEM3 |
|---|---|
| Target | Actin |
| Tissue Specificity | The antibody does not react with the epitope on the N-terminal end of actin. |
| Cellular Localization | Cytoplasmic |
Application
Tissue stained with conserved C-terminus specific Monoclonal Anti-Actin . Indirect immunofluorescence staining of human tongue frozen section with Monoclonal Anti-Actin, Clone No. AC-40 .
Application
ICC/IF analysis of Rat2 cells using Actin antibody [AC-40] at a dilution of 1:250 (red) and DAPI (blue).
Application
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK, and (9) MDCK lysates using Actin antibody [AC-40] at a dilution of 1:500.
Application
Immunohistochemical analysis of Agarose-embedded zebrafish embryo tail section , using Actin antibody at 1:200 dilution.| Application Notes | Dot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IHC-F: Use at an assay dependent dilution. IF: Use at a dilution of 1/2000 determined by indirect immunofluorescent staining of cultured human or chicken fibroblasts. IHC-P: Use at an assay dependent dilution. Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel?s or Bouin?s solution may also be used as fixatives. IHC-Fr: Use at an assay dependent dilution. WB: Use at a dilution of 1/500 using cultured human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Raw Ascites |
