| Product Name | Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [ACTN05 (C4)] to ActinBeta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. |
| Immunogen | Chicken gizzard actin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | ACTN05 (C4) |
| Host Species | Mouse |
| Tested Applications | IPWB |
| For IP: Use 2 μg/mg protein lysate. For WB: Use at a concentration of 0.5 μg/ml for 2 hours at room temperature. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDogPigRatDictyosteliumPhysarum PolycephalumRabbitBovine |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | ACTB 396526 beta Actin P60709 102630 RCJMB044h19 RCJMB04_4h19 LOC396526 60 |
|---|---|
| Target | Actin |
| Tissue Specificity | This antibody reacts with all six known vertebrate isoforms of actin. It also reacts with two cytoplasmic actins (beta, lambda) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acidresidues. |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | HeLa cells |
|---|---|
| Application Notes | For IP: Use 2 μg/mg protein lysate. For WB: Use at a concentration of 0.5 μg/ml for 2 hours at room temperature. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM Phosphate-buffered saline, pH7.4, containing 0.2%BSA and 0.09% sodium azide |
