Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] diluted at 1:500.Application
ADAR1 antibody [GT1066] detects ADAR1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: ADAR1 protein stained by ADAR1 antibody [GT1066] diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.Application
Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [GT1066] . Western blot analysis was performed using ADAR1 antibody [GT1066] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
ADAR1 antibody [GT1066] detects ADAR protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 5 % SDS-PAGE ADAR1 antibody [GT1066] dilution: 1:1000| Product Name | ADAR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to ADAR1 (adenosine deaminase, RNA-specific)This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ADAR1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT1066 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (80%), Rat (80%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Molecular Weight(MW) | 136 kDa (note) (5) |
|---|---|
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] diluted at 1:500.
Application
ADAR1 antibody [GT1066] detects ADAR1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: ADAR1 protein stained by ADAR1 antibody [GT1066] diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 μm.
Application
Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [GT1066] . Western blot analysis was performed using ADAR1 antibody [GT1066] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
ADAR1 antibody [GT1066] detects ADAR protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 5 % SDS-PAGE ADAR1 antibody [GT1066] dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , A375 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
