Application
Sample (30 µg of whole cell lysate) A: A431 B: HepG2 10% SDS PAGE adenosine kinase antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Adenosine kinase antibody detects ADK protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE Adenosine kinase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Adenosine kinase antibody detects ADK protein by western blot analysis. A. 50 µg mouse liver lysate/extract 10% SDS-PAGE Adenosine kinase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Adenosine kinase antibody at 1:200 dilution.Application
Adenosine kinase antibody detects Adenosine kinase protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Adenosine kinase antibody dilution: 1:500.Application
Adenosine kinase antibody detects Adenosine kinase protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Adenosine kinase antibody dilution: 1:500.| Product Name | Adenosine kinase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Adenosine kinase (adenosine kinase)This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Adenosine kinase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Rhesus Monkey, BovineChicken (83%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AK antibody ADK antibody adenosine kinase antibody adenosine 5'-phosphotransferase antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (3) |
Application
Sample (30 µg of whole cell lysate) A: A431 B: HepG2 10% SDS PAGE adenosine kinase antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Adenosine kinase antibody detects ADK protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE Adenosine kinase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Adenosine kinase antibody detects ADK protein by western blot analysis. A. 50 µg mouse liver lysate/extract 10% SDS-PAGE Adenosine kinase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Adenosine kinase antibody at 1:200 dilution.
Application
Adenosine kinase antibody detects Adenosine kinase protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Adenosine kinase antibody dilution: 1:500.
Application
Adenosine kinase antibody detects Adenosine kinase protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Adenosine kinase antibody dilution: 1:500.| Positive Control | A431 , HepG2 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
