Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000Application
Adenosine kinase antibody [N1C1] detects Adenosine kinase protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Adenosine kinase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Adenosine kinase (adenosine kinase)This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Adenosine kinase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (95%), Rat (94%), Xenopus laevis (86%), Chicken (88%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AK antibody ADK antibody adenosine kinase antibody adenosine 5'-phosphotransferase antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (4) |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000
Application
Adenosine kinase antibody [N1C1] detects Adenosine kinase protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Adenosine kinase protein stained by Adenosine kinase antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
