Mouse monoclonal [M58 + M73] to AdenovirusThe adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1A region encodes a series of related proteins (35 - 46 kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.
Immunogen
Adenovirus.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
M58 + M73
Host Species
Mouse
Tested Applications
IHCIHC-FIHC-P
Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/75 in an ABC method. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.:
Species Reactivity
Adenovirus
Concentration
1mg/ml
Target Information
Gene Synonyms
EIA
Target
Adenovirus
Cellular Localization
Nuclear.
Light Chain Type
kappa
Application
Application
IHC image using - Detection of adenovirus by IHC in human infected appendix
Positive Control
Suitable tissue control sections for Immunohistochemistry: Adenovirus infected tissue.
Application Notes
Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dliution of 1/50 - 1/75 in an ABC method. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C.