| Product Name | Adenovirus type 5 E1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [0.N.8] to Adenovirus Type 5 E1AThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen. |
| Immunogen | Tissue / cell preparationAdenovirus. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 0.N.8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIPWB |
| IF: Use at a concentration of 0.2 - 0.4 μg/ml. IHC-F: Use at a concentration of 2 - 4 μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, 10-20 min, followed by cooling at RT 20 min.IP: Use Protein A 2ug antibody/mg protein lysateWB: Use at a concentration of 0.5 - 1.0 μg/ml for 2 hrs at RT, Predicted molecular weight: 35-46 kDa. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Adenovirus |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Target | Adenovirus type 5 E1A |
|---|---|
| Tissue Specificity | recognises Adenovirus, Type 5 |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
| Positive Control | 293 cells or adenovirus infected cells and tissues. |
|---|---|
| Application Notes | IF: Use at a concentration of 0.2 - 0.4 μg/ml. IHC-F: Use at a concentration of 2 - 4 μg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, 10-20 min, followed by cooling at RT 20 min.IP: Use Protein A 2ug antibody/mg protein lysateWB: Use at a concentration of 0.5 - 1.0 μg/ml for 2 hrs at RT, Predicted molecular weight: 35-46 kDa. Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C short term. Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
