Application
Immunohistochemistry staining of human colon (paraffin sections) using anti-AGR2+AGR3 (clone AGR3.4)Application
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.| Product Name | AGR2+AGR3 antibody [AGR3.4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AGR3.4] to AGR2+AGR3AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.Entrez Gene (AGR2): 7p21.3Entrez Gene (AGR3): 7p21.1 |
| Immunogen | Purified human AGR3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AGR3.4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| WB: Use at 1 µg/mL. Positive Control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AG2, GOB-4, HAG-2, XAG-2 |
|---|---|
| Target | AGR2+AGR3 |
| Tissue Specificity | The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis. |
Application
Immunohistochemistry staining of human colon (paraffin sections) using anti-AGR2+AGR3 (clone AGR3.4)
Application
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.| Application Notes | WB: Use at 1 µg/mL. Positive Control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
