Application
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.| Product Name | AGR3 antibody [AGR3.2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AGR3.2] to AGR3AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.Entrez Gene: 7p21.1 |
| Immunogen | Purified human AGR3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AGR3.2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FWB |
| WB: Use at 1 µg/mL. Positive Control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | AGR3 |
|---|---|
| Tissue Specificity | The antibody AGR3.2 recognizes epitope QYSQALKKV within AGR3 (AG3) protein (19-20 kDa); secreted cytoplasmic protein which can serve as a marker of carcinogenesis. |
Application
Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.| Application Notes | WB: Use at 1 µg/mL. Positive Control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
