Application
Anti-AK5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AK5Application
HEK293T cells transfected with either RC222241 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK5 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.| Product Name | AK5 antibody [2A6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A6] to AK5Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. |
| Immunogen | Full length human recombinant protein of human AK5 (NP_777283) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2A6 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AK5, MGC33326, AK6 |
|---|---|
| Target | AK5 |
| Tissue Specificity | is specific for human AK5 (NP_777283). |
Application
Anti-AK5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AK5
Application
HEK293T cells transfected with either RC222241 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AK5 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
